摘要
目的：观察急性脑梗死患者的血小板ＣＤ６２Ｐ，红细胞内、外钙离子（Ｃａ２＋）浓度变化，旨在探讨血小板活化因子（ＰＡＦ）、Ｃａ２＋稳态失调对大脑的损害。方法：采用流式细胞免疫技术，以抗血小板单克隆抗体及钙离子荧光剂为分子探针，检测了４０例急性脑梗死患者，３６例健康查体者的血小板ＣＤ６２Ｐ、红细胞内钙（ＩＥＣａ２＋）及血清钙（ＳＣａ２＋）浓度。结果：脑梗死患者 ＣＤ６２Ｐ、ＩＥＣａ２＋明显高于对照组，ＳＣａ２＋则明显低于对照组，两组比较差异非常显著（ｐ＜ ０．０１）。 ＩＥＣａ２＋随着 ＣＤ６２Ｐ的增高而增高，两者是正相关（ｐ＜ ０．０５）；而血清 Ｃａ２＋则随着ＩＥＣａ２＋的增高而降低，两者呈负相关（ｐ＜０．０５）。ＣＤ６２Ｐ、ＩＥＣａ２＋及ＳＣａ２＋与病情程度、梗塞面积大小相关，病情重、面积大者ＣＤ６２Ｐ、ＩＥＣａ２＋增高显著，ＳＣａ２＋降低亦显著。结论：ＰＡＦ在Ｃａ２＋稳态失调中起着重要作用； ＰＡＦ及Ｃａ２＋稳态失调与脑梗死的发生发展密切相关。
Objective:The level of platelet active factor(PAF)、intraerythrocytic calcium(IECa2+) and extraerythrocytic calcuium(EECa2+)in acute cerebral infarction were observed to investigate the effects of PAF and calcium homeostasis disorder on cerebral damage. Methods:CD62 of platelet、IECa2+ and serum Ca2+ (SCa2+) were detected by flow cytometric immunology in 40 patients with acute cerebral infarction and 36 normal persons. Antiplatelet monoclonal antibody and calcium-Fluorescenin-3/AM were used as molecular probe. Results:CD62P and IECa2+ in patients with acute cerebral infarction were significantly were than that of control group,while SCa2+ of the patients were markedly lower than that of control group(p＜0.01).IECa2+ level were significantly positive correlated with CD62P,while Sca2+ level were significantly passively correlated with CD62 P(p<0.05).CD62P and IECa2+ level were ralated with clinical conditon and the size of infarction area. CD62P and IECa2+ level were markedly increase in the patients of serious condition and large infarction area. Conclusion:PAF plays an important role on the disorder of calcium homeostasis. PAF and the disorder of calcium homeostasis were closely related to the attack and development of cerebral infarction.
出处
《河南实用神经疾病杂志》
2001年第2期4-5,共2页
Henan Journal of Practical Nervous Diseases
关键词
急性脑梗死
CD62P
红细胞内钙
血清钙
钙稳态失调
Acute cerebral infarction CD62P Intraerythrocytic calcium Serum calcium Calcium homeostasis disorder
