摘要
目的 探讨Ｌ－精氨酸抗血管内膜增生的作用机制是否与影响球囊损伤后血管中细胞周期调控基因、细胞周期依赖性激酶２（ＣＤＫ２）、细胞周期蛋白 Ｅ（ＣｙcｌｉｎE）和增殖细胞核抗原（ＰＣＮＡ）的表达有关。方法 21只大鼠随机分为Ｓ组（假手术组），Ｃ组（球囊损伤对照组）及Ｌ组（球囊损伤＋L－精氨酸组）各组 ｎ= ７。术后 １４ｄ采血测血浆一氧化氮（ＮＯ）水平，并处死大鼠取其胸主动脉，测新生内膜面积，采用免疫组化和计算机图像分析法测ＣDK2、ＣｙclｉｎＥ和PCＮＡ的表达水平。结果 Ｃ组的血浆ＮＯ水平明显低于S组，与Ｃ组相比，Ｌ组的血浆N０水平增高（Ｐ＜0．０１），新生内膜面积减少５９．１％（Ｐ＜０．01），ＣＤＫ2、ＣｙcｌｉｎＥ及PCＮＡ的阳性表达指数分别降低３６．１％，４６．３％和７６．２％((Ｐ均＜０．01）。结论 Ｌ-精氨酸抑制球囊损伤后血管中ＣＤＫ2、ＣｙｃlinＥ及ＰＣＮＡ的高表达可能是其抑制血管损伤后内膜增生的分子机制之一。
Objective To determine whether the anti-proliferation effect of L-arginine is due to inhibiting the expression of cyclin dependent kinase-2 (CDK2)、 CyclinE and proliferation cell nuclear antigen (PCNA) in blood vessel after balloon injury. Methods Rats were randomized into three groups: Group S (sham operation group), Group C (balloon injury control group) and Group L (balloon injury + L-arginine group). After 14 days, blood samples were collected for biochemical studies, and the thoracic aortas were harvested for immunohistochemistry. The expression of CDK2、 CyclinE and PCNA were measured by means of computer image analayzer. Results The levels of plasma nitric oxide (NO) in group C were significantly lower than those in group S. Compared with group C, the levels of plasma NO increased (P < 0.01), the neointima area reduced by 59. l% (P < 0.01) and the positive expression indexes of CDK2、CyclinE and PCNA decreased by 36. l%, 46.3% and 76.2% respectively in group L (P all < 0.01). Conclusion Inhibitory effect of L-arginine on CDK2、CyclinE and PCNA high expression may be one of the mechanisms of the anti-proliferation effect of L-arginine after balloon injury.
出处
《中国介入心脏病学杂志》
2001年第2期104-106,共3页
Chinese Journal of Interventional Cardiology
基金
河南省科技攻关项目(971200100)
