摘要
应用血型抗原A、B、H的单克隆抗体及先进的SABC(Streptavidin-biotin Complex)技术对常规处理的51例食管癌组织切片进行免疫组织化学研究.结果表明:该方法为脉管内皮细胞提供了稳定而清晰的染色.能更加客观地观察食管癌组织内部间质及周围组织中的癌栓.其显示脉管侵袭的阳性率为43.14%.明显高于HE染色的23.53%(P<0.01)。食管癌组织侵袭脉管与癌组织的分化程度、生长方式及淋巴结转移等有密切关系(P<0.05)。
Monoclonal antibodies to ABH isoantigens and an advanced SABC staining technique were used tostudy the immunohistochemistry of 51 sections of formalinembedded esophageal tissues from patientswith advanced squamous cell carcinomas of the esophagus. The results indicated that this method pro-duced consistent and specific staining of endothelium cells in the vessels,could made an objective obser-vation of the mesenchyma in the tissue of esophageal carcinoma and the cancerous emboli in the pe-ripheral tissues. The positive rate of vascular invasion by using monoclonal antibodies and SABC stain-ing technique (43.14%) was higher than that by HE staining sections(23.53%) (P<0. 01). The re-sults showed vascular invasion of esophageal cancer tissues had close relation with their degrees of dif-ferentiation,growth patterns and metastasis to lymph nodes(P<0. 05).
出处
《河南医科大学学报》
1991年第3期203-206,共4页
Journal of Henan Medical University
关键词
食管肿瘤
脉管
免疫组织化学
endothelial cells
immunohistochemistry
ABH isoantigens
monoclonal antibodies
vascular (lymphatic and/or blood vessel)invasion
carcinoma of the esophagus
