摘要
目的 :探讨迷走神经作为LPS信息由外周传入中枢的桥梁。方法 :实验组为膈下迷走神经切断并给予LPS组 ,3个对照组为假手术生理盐水组 ,假手术LPS组 ,膈下迷走切断生理盐水组。测体温用数字体温检测仪测定大鼠肛温。c Fos蛋白表达用免疫组织化学法检测。结果 :实验组肛温变化值与假手术LPS组相比明显降低 ,差异显著 ,P <0 0 5 ;与迷走神经切断生理盐水对照组相比明显增高 ,P <0 0 5。c Fos蛋白表达 :实验组PVN和NTS中c Fos阳性细胞百分率与假手术LPS组相比明显降低 ,差异显著 ,P <0 0 1;与迷走神经切断生理盐水对照组相比明显增高 ,P <0 0 1。结论 :迷走神经是外周LPS信息向脑传递的途径之一。
Objective:To study the possibility that responses of fever and c Fos expression in rat PVN and NTS to intraperitoneal administration of LPS are mediated by vagal afferents.Methods:Rectal temperature was detected by digital temperature detecting instrument.c Fos expression was detected by immunohistochemistry staining.Results:The rectal temperature change value in vagotomy LPS group was significantly decreased compared with that in sham LPS group,and there was striking difference between them,P<0 05.It was increased significantly compared with that in vagotomy NS group,P<0.05.The percentage of c Fos positive neurons in rat PVN and NTS in vagotomy LPS group was significantly decreased compared with that in sham LPS group,and there were distinct difference between them,P<0.01.It was strikingly increased compared with that in vagotomy control group,respectively,P<0.01.Conclusion:The results indicate that vagus nerve is one of the pathways of peripheral LPS signal communicating to CNS.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第4期201-203,206,共4页
Chinese Journal of Immunology
