摘要
目的 探讨软组织恶性淋巴瘤的病理特点及基因诊断方法。方法 在组织学诊断基础上 ,辅以免疫组织化学染色法 ,利用PCR技术检测石蜡切片标本IgH及TCRβ基因重排 ,予以确诊。 结果 将病变不够典型、淋巴瘤诊断依据不充分或诊断意见不一致的 4例病例确诊为软组织恶性淋巴瘤。发生部位为大腿、小腿、前臂及肩胛下 ,均为B细胞性弥漫性非霍奇金淋巴瘤。免疫组化 :CD45 (LCA) (+ ) ,CD2 0 (L2 6 ) (+ ) ,CD45RO(UCHL1) (- ) ,Mac387(- ) ,S 10 0 (- ) ,NSE(- ) ,desmin(- )。PCR :IgH基因重排 (+ ) ,TCRβ基因重排 (- )。 结论 软组织恶性淋巴瘤组织学形态复杂多变 。
Objective To explore the clinicopathological features of lymphoma in the soft tissues and its diagnostic significance of genetic analysis. Methods Histopathological observation, immunohistochemical staining and detection of clonal genetic rearrangements by PCR were used to analyze 4 atypical cases of lymphoma primarily occurred in the soft tissues. Results All the 4 cases were diffuse B cell non-Hodgkin's lymphoma with immunophenotypes of CD45(+), CD20(+), CD45RO(-), Mac387(-), S 100(-), NSE(-) and Desmin(-). Genotyping showed a clonal rearrangement of IgH gene and germline pattern of TCRβ gene. Conclusions Histopathological changes of lymphoma primarily occurred in the soft tissues are often variable. It is extremely effective for the diagnosis of this rare lymphoma to use immunophenotyping and genotyping methods.
出处
《诊断病理学杂志》
CSCD
2001年第2期71-73,I022,共4页
Chinese Journal of Diagnostic Pathology
基金
国家自然科学基金!(39770 912 )
军队医药卫生基金!(96Q47)资助项目
