摘要
目的 研究腺病毒介导的脑源性神经营养因子 (BDNF)基因转移对脑损伤后细胞凋亡的影响。 方法 将重组腺病毒载体 4μl注入承受单侧大脑皮质重锤打击伤的海马区 ,对照组注射病毒缓冲液。伤后 3h、1,3,7,14d利用免疫组化单标和 (或 )双标染色、原位杂交 -免疫组化双标染色、DNA末端原位标记以及流式细胞仪等方法 ,检测伤侧大脑皮质、海马区BDNF及凋亡相关信号表达的改变。 结果 与对照组相比 ,注射病毒载体组动物术后 3,7d海马CA1、CA3区BDNF神经元显著增多 ,而凋亡细胞显著减少 (P <0 .0 1) ;表达BDNF的神经元较少并同时表达凋亡相关信号。 结论 腺病毒介导的BDNF基因转移对海马神经元具有保护作用。
Objective To investigate the effects of gene transfer of adenovirus-mediated brain-derived neurotrophic factor (BDNF) on apoptosis after traumatic brain injury. Methods Adult Wistar rats experienced a weight-drop strike on the right cerebral cortex, and then 4 μl recombinant adenovirus vector (RAV) and 4 μl virus buffer were injected into the hippocampus in the expermental group and in the controls, respectively. Immunohistochemistry and/or in situ hybridization, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and flowcytometry were used to determine the expressions of BDNF and apoptosis-associated signals in the hippocampus and cerebral cortex areas at 3 hour, 1, 3, 7 and 14 days following injury. Results Compared with the controls at 3 and 7 days after injury, BDNF positive cells increased significantly, while the apoptotic cells decreased significantly in CA1 and CA3 areas in the RAV group (P<0.01). Meanwhile, the cells expressing BDNF showed less apoptotic signals. Conclusions RAV-mediated BDNF gene transfer protects hippocampal neurons through up-regulating BDNF expression and inhibiting programmed cell death.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2001年第5期264-268,共5页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目! (3 6970 740 )
