摘要
目的 血 -视网膜屏障破坏 (Blood -retinalBarrierBreakdown ,BRBB)是许多眼底病的共同病理改变。本研究的目的是探讨能否应用伊凡思蓝 (Evansblue ,EB)作为示踪物定量诊断BRBB。方法 实验动物选用体重约 2 0 0克的雄性Sprague -Dawley大鼠。首先应用EB测定由血管内皮生长因子 (vascularendothe lialgrowthfactor ,VEGF)引起的视网膜血管渗漏。然后用EB测量早期糖尿病导致的BRBB。以甲酰胺提取视网膜中的EB ,用分光光度计测量提取液中的EB浓度。最后根据视网膜重量标准化视网膜内的EB含量。结果 VEGF处理眼视网膜内的EB含量显著高于未处理眼视网膜内的EB含量 (P <0 .0 0 0 1)。早期糖尿病大鼠视网膜内的EB含量亦显著高于正常对照组视网膜内的EB含量 (P <0 .0 5)。
Objective Blood-retina barrier breakdown is a common pathological change of many retina diseases. The purpose of this study is to test whether the Evans blue dye can be used to quantitatively checking the retina blood vessel leakage. Methods Male Sprague-Dawley rats weighing 200 grams were used in this study. The VEGF induced retinal blood vessel leakage was checked with Evans blue dye. Then the blood—retina barrier breakdown of 1 week diabetic animals was quantified with Evans blue. The dye was extracted from retina by formamide and the extraction was checked with spectrophotometer. Evans blue leakage was normalized against wet or dry retina weight. Results The retinal Evans blue content of eyes treated with VEGF was remarkably higher than that of the controls (n=17, P <0.0001). And the eyes of 1 week diabetic duration animals have more Evans blue dye than that of the normal controls ( P <0.05).Conclusion Evans blue dye is a sensitive tracer in quantitatively diagnosing the blood retina barrier breakdown.
出处
《济宁医学院学报》
2001年第1期9-11,共3页
Journal of Jining Medical University
基金
国家留学基金资助!(9813716 8)
