摘要
目的 自发性高血压大鼠 (SHR)胸主动脉外膜胶原分布及含量的变化。方法 天狼猩红染色法观察不同周龄组 (4W、8W、2 4W )大鼠胸主动脉外膜胶原的分布 ,氯胺T氧化法测定不同周龄组胸主动脉外膜胶原含量 ,组织块贴片法进行血管外膜成纤维细胞的培养 ,并采用细胞免疫化学法检测血管外膜成纤维细胞Ⅰ、Ⅲ型胶原的表达。结果 大鼠胸主动脉胶原主要分布于血管外膜 ;自发性高血压大鼠胸主动脉外膜胶原含量在 8周、2 4周高于对照京都种Wistar大鼠 (WKY) ;血管外膜成纤维细胞Ⅰ、Ⅲ型胶原免疫细胞化学染色呈阳性。结论 胸主动脉外膜胶原的沉积可能参与高血压血管重塑过程 ,血管外膜成纤维细胞可合成Ⅰ。
Objective: To study collagen distribution and its quantitative variation in the aortic adventitia of spontaneously hypertensive rats Methods: Sirus red stain was used to observe collagen distribution in aortic adventitia;Chloramine\|T oxidation assay was employed to quantitate the collagen of aortic adventitia;Immuno\|histochemical staining was adopted to detect type Ⅰ、Ⅲ collagen synthetic characteristic of vascular adventitial fibroblasts. Results: Collagen of rat aoritc wall mainly distributes in adventitia;SHR shows more collagen deposition in adventitia than WKY at the age of 8weeks and 24 weeks;The immuno\|histochemical staining of type Ⅰ、Ⅲ collagen in vascular adventitial fibroblasts is positive. Conclusions: All those results indicate that vascular adventitia may be involved in hypertensive vascular remodeling.and vascular adventitial fibroblasts can synthesize collagen type Ⅰ and Ⅲ.
出处
《高血压杂志》
CSCD
2001年第1期50-52,共3页
Chinese Journal of Hypertension
基金
卫生部基金资助项目
