摘要
试验比较了来自东北、华北等地小麦根腐病菌共30个菌株,从中已筛选出毒力强而稳定的菌株(002号)。初步明确采用改良Fries加小麦健叶汁的液体培养基培养根腐茵,每天振荡4次,每次20min,培养15d后的培养物滤液,在5℃下可保存2个月,用15磅高压灭菌30min及煮沸浓缩均不影响毒素的作用。在几种Hs—毒素制剂中,以粗提物制剂的毒素作用最强,病叶提取液和培养滤液次之。应用根冠细胞测定法评价Hs—毒素的作用比用抑制种子根伸长的种苗“萌发包”法更为灵敏,精确,快速。测定小麦离体:浪冠细胞的最佳条件是:黑暗25℃下处理6h;根冠细胞浓度为500—1000个/ml;粗提毒素稀释液pH6—7。
Thirty isolates of Helminthosporium sativum (Cochliobolus sativus) from Northeast and North China were comparied.And isolate No.002 was selected with its high virulence and stability.The experimental results suggested that pathotoxin preparations from H.sativum cultured with Modified Fries Liquid medium added with health leaf extract of wheat by means of being shaken four times one day (20min/time) could maintain their pathoactivity for two months at 5℃.The role of HS-toxin was not affected after sterilizing 30℃ min in autoclave (121℃, 15Ib/m) or boiling for concentration. The effect and pathoactivity of the crude HS-toxin preparation was the strongest among sevecal preparations used.
The root cap cell bioassay used for evaluating the role of HS-toxin was more exact and rapid than inhibiting root growth method, and the suitable conditions of the former bioassay were 500-1000/ml root cap cells treated for 6hrs under dark 25℃ in the crude Hs-toxin of pH value being 6-7.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1991年第2期54-59,共6页
Journal of Hebei Agricultural University
关键词
小麦
根腐病
致病毒素
组织培养
Helminthosporium sativum
wheat
pathotoxin
tissue culture
