摘要
观察木黄酮对心肌成纤维细胞 (CF)增殖的影响 .采用培养的新生大鼠CF ,以 [3 H]TdR参入率作为细胞增殖指标 ;流式细胞仪分析细胞周期 .结果显示木黄酮 (0 .5～ 50 μmol·L- 1)具有浓度依赖性地抑制 2 .5%胎牛血清刺激CF增殖的作用 ,并将CF细胞周期阻抑在G2 /M期 ;
To study the effect of genistein on the proliferation of cardiac fibroblasts(CF), CFs were cultured from neonatal rat hearts, DNA synthesis of the cells was determined by incorporation of [ 3H]TdR into DNA, the cell cycle was measured by flow cytometric analysis. Genistein(0.5-50 μmol·L -1 ) attenuated 2.5% fetal calf serum-induced proliferation of CF in concentration-dependent manner. Genistein(50 μmol·L -1 ) arrested CF cell progression at G 2/M phase. The results suggest that genistein be a potential substance for treatment of cardiac fibrosis.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2001年第2期159-160,共2页
Chinese Journal of Pharmacology and Toxicology
