摘要
目的 在人外周血细胞中筛选新的线粒体DNA(mtDNA)大片段缺失突变 ,探讨mtDNA大片段缺失突变与衰老的关系。 方法 以人外周血细胞DNA为模板 ,进行聚合酶链反应 (PCR)扩增 ,产物克隆、测序。用PCR半定量方法测定 <6 0岁组和≥ 6 0岁组中突变型与野生型的比例。 结果 在人外周血细胞中发现 1条长达 13 1kb的mtDNA缺失突变 ,<6 0岁组和≥ 6 0岁组突变型与野生型的比例 ,差异有显著性。 结论 该突变以前未见报道 ,可能是一种与衰老有关的缺失突变。
Objective To screen a new deletion in mitochondria(mtDNA) fragment in human peripheral blood cells, and study the relationship between the mtDNA deletion and the aging. Methods DNA extracted from human peripheral blood cells was used as PCR templates. The PCR products were cloned and sequenced. Semi quantitative PCR was also used in detecting the ratio of deleted mtDNA to wild type mtDNA. Results There was a 13 1 kb mtDNA deleted in human peripheral blood cells, and the deletion ratio between <60 age group and ≥60 age group was significantly different. Conclusions To our knowledge,this deletion has not been reported before and was the longest deleted fragment in human mitochondrial genome and it might be an aging related deletion mutation.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第2期120-123,共4页
Chinese Journal of Geriatrics
