摘要
目的 探讨中药汉防己甲素 (Tetrandrine)对单侧肾切除加两次注射阿霉素肾小球硬化大鼠肾皮质IV型胶原基因表达的作用。方法 将实验动物分为 :正常对照组、汉防己甲素治疗组、氨氯地平治疗对照组和模型组 ,于第 12周留取尿标本。肾组织病理学检查、用Northernblot杂交检测皮质IV型胶原基因表达mRNA表达。结果 汉防己甲素治疗组和氨氯地平治疗对照组 ,肾病理损伤减轻 ,肾皮质IV型胶原mRNA表达下调。结论 汉防己甲素可延缓肾小球硬化。
Objective: To investigate the effect of tetrandrine on typeIV collagen mRNA in glomerularsclerosis rats. Methods: Sham operative rats were groups 1 as control. Glomerular sclerosis rat models, made by unilateral renctomy plus adriamycin injection in twice, were divided into group 2 (tetrandrine), group 3 (amlodipine) and group 4 (untreated model). After 12 weeks, urine was collected and renal histology was examined while typeIV collagen mRNA expression of kidney cortex was assessed by Northern blot analysis. Results: Pathological changes of the group 2 and 3 were improved compared with the group 4. The expression of typeIV collagen mRNA in the group 2 was lower than that in the group 4. Conclusion: Tetradrine can down-regulate typeIV collagen mRNA expression, and attenuate glomerular sclerosis.
出处
《泰山医学院学报》
CAS
2001年第1期18-19,共2页
Journal of Taishan Medical College
基金
上海市高层次中西医结合临床科研人才资助项目
