摘要
番茄种子经搭载返地卫星宇宙飞行３５５ｈ，回收后地面种植，选择３个同工酶有差异的宇宙飞行处理群体植株进行ＲＡＰＤ检测。结果表明：在５０个供试引物中，有１８个扩增出了ＤＮＡ带，共有１６６条，其大小在２００～２０００ｂｐ之间。与地面对照相比，宇宙飞行处理植株的ＤＮＡ在５个引物出现差异，其突变的程度分别为４．５％、１．３％和３．２％。
The dry tomato seeds were carried in a recoverable satellite lasting 355 hours in space flight.They were planted on the ground.Three lines showing difference in peroxidase isozyme were used for RAPD analysis.Among 50 primers used in this experiment,18 primers generated 166 polymorphic DNA bands with size from 200 bp to 2 000 bp.Totally,the rates of DNA mutation of these three lines tested are 4.5% , 1.3 % and 3.2% ,respectively.
出处
《园艺学报》
CAS
CSCD
北大核心
1999年第1期33-36,共4页
Acta Horticulturae Sinica
基金
国家高技术航天领域863-2支持课题
