摘要
目的探索“胃经-脑肠肽-免疫调节网络”在针刺调控胃肠功能中的多因素协调作用及可能的内在相互联系。方法将大鼠随机分成正常对照组、非经非穴组、足三里组、免疫抑制组、足三里+免疫抑制组,同步观察电针后某些脑肠肽及免疫功能的变化。应用放射免疫法测定脑垂体和外周血中P物质(SP)、血管活性肠肽(VIP)的含量;应用流式细胞仪技术,通过微量全血直接免疫荧光染色法测定外周血T细胞亚群以反映细胞免疫功能;应用红细胞C_(3b)受体-酵母菌花环试验和红细胞-IC花环试验检测红细胞免疫功能。结果电针刺激足三里穴后正常大鼠外周血的CD4+,RBC-C_(3b),RR,RBC-ICR均明显升高(P均<0.01),CD8+无显著变化(P>0.05);脑垂体和外周血中SP,VIP的含量增加(P均<0.01);且CD4+与SP,VIP含量的变化(r=0.744,P<0.05;r=0.738,P<0.05;r=0.822,P<0.05:r=0.848,P<0.05)、CD4+和RBC-C_(3b)RR呈显著正相关(r=0.719,P<0.05),免疫抑制组大鼠CD4+,RBC-C_(3b)RR明显低于正常对照组(P均<0.05),CD8+无显著变化(P>0.05);脑垂体和外周血中SP,VIP的含量减少(P均<0.01)。足三里+免疫抑制组CD4+(P<0.01),RBC-C_(3b)RR(P<0.01)以及脑垂体和外周血中SP,VIP的含量(P均<0.01)与免疫抑制组有差异,与正常对照组相比无显著差异(P>0.05),非经非穴组的各项指标与正常对照组相比无显著差异(P>0.05)。结论电针刺激足三里穴在提高正常大鼠及免疫抑制模型大鼠的细胞免疫功能和红细胞免疫粘附功能的同时可引起多种脑肠肽的变化。提示针刺胃经对神经-内分泌-免疫网络具有调节作用,这些脑肠肽又作为调节因子,对全身免疫系统发生影响,同时免疫网络之间、调节因子之间也存在着相互调节和制约,形成一个复杂、有机的网络体系,从而实现针刺对机体的双向性良性调衡作用。
AIM To investigate regulatory effects of electroacupuncture (EA) at Tsusanli (He-Sea Point, St 36) on changes in ir-SP and ir-VIP content in rats pituitary gland and peripheral blood as well as on their cellular immunity and erythrocytic immunity; and to explore the mechanism, or the coordinating effects of 'the stomach channel brain-gut immune network' and the possible inter relationship between the many factors involved in the coordination. METHODS The rats were randomized into 5 groups, including the control, the Tsusanli, the immunosuppressive model, the non-acupoint group and the Tsusanli +immunosuppressive groups. Changes in substance P (SP) and vasoactive intestinal peptide (VIP) contents in the pituitary gland and peripheral blood were determined by radioimmunoassay. With micro-whole-blood direct immunofluorescence staining and flow cytometry, Tlymphocytic subgroups were assayed to reflect the cellular immunity. Erythrocytic immunity was assayed by red blood cell C_(3b)receptor rosette (RBC-C_(3b)RR) and rod blood cell immune complexes rosette (RBC-ICR). RESULTS Ir-SP and ir-VIP content in the pituitary gland and peripheral blood and CD4+and RBC-C_(3b) RR in the peripheral blood of the rats of the immunosuppressive group were significantly lower than that of the control group (P<0.01, P<0.01, P<0.01 and P<0.05 respectively). There was no significant change in CD8+ content (P>0.05) of all the groups. There was no significant difference between the indexes of the Tsusanli +immunosuppressive group and that of the control group (P>0.05). After EA at Tsusanli. a significant increase was observed in ir-SP and ir-VIP content in the pituitary gland and peripheral blood, CD4+, RBC-C_(3b)RR, RBC-ICR in the peripheral blood of the normal rats (P<0.01) whereas CD8+did not change significantly (P>0.05). SP and VIP contents in the pituitary gland and the peripheral blood increased (P<0.01). Besides, the change in CD4+content and that in SP and VIP contents were positively correlated with each other (r=0.744, P<0.05; r=0.738, P<0.05; r=0.822, P<0.05; r=0.848, P<0,05)and so were that between CD4+and RBC-C_(3b) RR (r=0.719, P<0.05). No significant difference was observed in the indexes between non-acupoint group and the control. CONCLUSION EA at Tsusanli (He-Sea Point, St 36) can improve the cellular immunity and erythrocytic immunoadhesiveness of normal rats and immunosuppressed rats. The chang·s in SP and VIP contents in the pituitary gland and peripheral blood indicate that EA can improve immunity of the body. The mechanism of this might be related to the increased synthesis and release of gastrointestinal hormones, which in turn affects the nerve-endocrine-immunoregulation network by means of these immuno-transmitters.
出处
《世界华人消化杂志》
CAS
2001年第3期279-283,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.39970888
