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简易法冻存脐血造血干细胞的研究 被引量：3

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摘要 目的 :为脐血造血干细胞 (CBHSC)的冻存建立简便、经济且有效的方法。方法 :采用 5 %二甲基亚砜 (DMSO)加6 %羟乙基淀粉 (HES)为冷冻保护剂 ,不用程控降温装置 ,直接置 CBHSC于 - 80℃冰箱中降温 ,后分 - 80℃冰箱组和液氮组保存 1周至 3个月。结果 :冻存 3个月后 ,单个核细胞 (MNC)和粒 -单系造血祖细胞 (CFU- GM)的回收率及台盼兰拒染率在 - 80℃冰箱保存组分别为 (92 .9± 0 .8) % ,(82 .2± 19.6 ) % ,(82 .1± 1.4) % ;在液氮保存组分别为 (93.0± 1.2 ) % ,(93.6± 2 2 .2 ) % ,(85 .6± 1.5 ) %。结论 :这两种简便、经济的方法能很好地保存 CBHSC的造血潜能 ,能为脐血移植冻存CBHSC。
作者 钟卫一 牛威林 赖永榕 卢玉英 宁自觉
机构地区 广西民族医院 广西医科大学笫一附属医院
出处 《广西医科大学学报》 CAS 2001年第1期66-68,共3页 Journal of Guangxi Medical University
基金 广西壮族自治区教育厅自然科学基金!资助 (96 0 48)
关键词 脐血造血干细胞 冻存 二甲基亚砜 羟乙基淀粉 CBHSC
分类号 R457 [医药卫生—治疗学] R318.52 [医药卫生—生物医学工程]
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参考文献8

  • 1[1]Stiff PJ,Koester AR,Weidner MK,et al.Autologous bone marrow transplan tation using unfractionated cells cryopreserved in dimethylsulfoxid and hydroxye thyl starch without controlled-rate freezing.Blood 1987,70:974-978.
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同被引文献12

  • 1周雪琴,丁小萍.自体造血干细胞回输中二甲亚砜致过敏性休克1例的紧急救护[J].解放军护理杂志,2004,21(6):91-92. 被引量：11
  • 2Inada H.Cryopreservation and engraftment potential of peripheral blood stem cells:pediatric experience[J].Kunune Med J,2001,48(2):151.
  • 3Hal E,Broxmeyer,Edward F,et al.High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years[J].PNAS,2003,100:645-650.
  • 4U-pratya Y,Boonmoh S,Promsuwicha O,et al.Collection and processing of umbilical cord blood for cryopreservation M[J].J Med Assoc Thai,2003,86(11):1055-1062.
  • 5Kawano Y,Lee CL,Watanabe T,et al.Cryopreservation of mobilized blood stem cells at a higher cell concentration without the use of a programmed freezer[J].Ann Hematol,2004,83(1):50-54.
  • 6Inada H.Cryopreservation and engraftment potential of peripheral blood stem cells:pediatric experience[J].Kurume Med J,2001,48(2):151-157.
  • 7Syme R,Bewick M,Stewart D,et al.The role of depletion of dimethyl sulfoxide before autografting:on hematologic recovery,side effects,and toxicity[J].Biol Blood Marrow Transplant,2004,10(2):135-141.
  • 8Clapisson G,Salinas C,Malacher P,et al.Cryopreservation with hydroxyethylstarch (HES)+dimethylsulfoxide (DMSO) gives better results than DMSO alone[J].Bull Cancer,2004,91(4):E97-E102.
  • 9Kudo Y,Minegishi M,Itoh T,et al.Evaluation of hematological reconstitution potential of autologous peripheral blood progenitor cells cryopreserved by a simple controlled-rate freezing method[J].Tohoku J Exp Med,2005,205(1):37-43.
  • 10黄友章,沈建良,杨平地,吴南海,唐湘凤,宫立众,岑坚,王立新,王宁,郑培浩.外周血造血干细胞不同保存方法的比较[J].中国应用生理学杂志,2008,24(1):125-128. 被引量：10

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广西医科大学学报
2001年 第1期

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