摘要
幽门螺杆菌 (Helocobacterpylori,Hp)是慢性胃炎、消化性溃疡的主要病原菌 ,为分析Hp超声上清经微粒包裹后对小鼠的口服免疫效果 ,采用ELISA法检测血清、唾液、肠粘液的抗体改变情况 ,ELISPOT法分析派伊尔氏结 (PP结 )抗原特异性抗体形成细胞 (ASC)的数量增减。发现微粒包裹型Hp疫苗免疫后可诱导较高的唾液sIgA水平和肠道sIgA反应 ,PP结抗原特异性抗体形成细胞 (ASC)
Helicobacter pylori is a major cause of chronic antral gasritis and peptic ulcer diseases.To analysis the mucosal immune responses in BALB/c Mice by oral immunization with H.pylori lysates encapsulated in copolymers of poly(D,L-lactide) and polyethylene glycol(PLEA) microparticles,we assayed antibody production in salivary and gut washing by ELISA and antibody secreting cells in Peyer's Patches(PP) by ELISPOT.Oral immunizations induced more significant H.pylori-specific intestinal IgA response as well as salivary IgA response than those detected with unencapsulated soluble antigen(P<0.05).The presence of antibody-secreting cell in PP was correlated with IgA level in gut washing .These data suggested that PLEA-Hp could induce H.pylori-specific mucosal immune responses in vivo and locally synthesized specific sIgA antibodies.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第3期10-12,共3页
Chinese Journal of Zoonoses
基金
国家"九五"重点科技攻关项目 !(96 -90 1-0 1-5 4)
