摘要
根据 C57BL6小鼠 Y染色体重复序列 1 4 5C5的碱基顺序 ,设计并合成一对引物 ,运用 PCR扩增昆明白小鼠植入前胚胎卵裂球 DNA,以确定其性别 .共对 1 0 8枚活检胚胎的相应卵裂球进行了性别诊断 ,获雄性胚 4 6枚 ,雌性胚 62枚 ,移植后分别获雄性仔鼠 4只 ,准确率 1 0 0 % ( 4/4 ) ,雌性仔鼠 9只 ,准确率 70 %( 9/1 3) .本研究结果表明小鼠 Y染色体重复序列 1 4 5C5的碱基顺序在 C57BL6小鼠和昆明白小鼠中基本一致 ;为农牧业动物进行性别选择和运用
In order to determine the sex of mouse embryo, 1 or 2 blastomeres were biopsied from Kun ming white mouse preimplantation embryo at 4 8 cells stage. The gDNA of the single blastomere was abstracted. According to the base sequence of 145C5, a repititive sequence of Y chromosome of mouse C57BL6, a pair of primer were asigned and synthesized. The gDNA was amplified using these primers. 108 mouse preimplantation embryos were sexed via this technique. 46 male embryos and 62 female embryos were transfered into five pseudopreganant mothers respectively. 4 male litters and 9 female litters were obtained. The diagnosis positive rate was 100%(4/4) and 70% (9/13)respectively. The result of PCR indecated that there was no difference between the repititive sequence of Y chromosome in mouse C57BL6 and Kun ming white mouse. The technique developed in this study might be further used for preimplantation genetic diagnosis of single gene defects.
出处
《生命科学研究》
CAS
CSCD
2001年第1期48-51,共4页
Life Science Research
基金
湖南省科委基金资助项目 !(10 13-8)
湖南省卫生厅基金资助项目!(0 0 0 2 )
关键词
植入前遗传学诊断
胚胎
聚合酶链反应
性别鉴定
小鼠
preimplantation genetic diagnosis
embryo
polymerase chain reaction
sex determination
blastomere
microbiopsy
mouse
