摘要
基于PC机和Linux操作系统 ,利用Phred/Phrap/Consed软件和Blast软件 ,构建了核酸序列大规模自动分析系统 .该套系统可自动完成从测序峰图向核酸序列的转化、载体序列去除、序列自动拼接、重复序列鉴定以及序列的相似性分析 ,可加速对大规模测序数据的分析和利用 .
More and more DNA sequences have been obtained since the start-up of human genome project. Powerful system is badly needed for data mining on these DNA sequences. Based on a personal computer and Linux operating system, the Phred/Phrap/Consed software and Blast software were used to construct a platform for batch analysis of the sequences, including identifying raw DNA sequence from chromatogram file, vector sequence removing, contig analysis (sequence assembly), repeat sequence identifying and sequence similarity analysis. Result demonstrated that this robust platform could accelerate data analysis for large-scale DNA sequencing.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第2期263-266,共4页
Progress In Biochemistry and Biophysics
基金
军事医学科学院科技创新研究启动基金! (990 5 10 5 )
国家"86 3"项目! (86 3 10 2 10 0 4 0 4)
国家杰出青年科学基金!(396 2
关键词
PC机
LINUX操作系统
软件
生物信息学
大规模测序
核酸序列
personal computer
Linux operating system
Phred/Phrap/Consed software
Blast software
bioinformatics
large-scale DNA sequencing
