摘要
为获得具有生物学活性的hFKBP5 2 ,来筛选新型的促神经再生药物 .采用半巢式、桥联PCR及亲和层析方法 ,从人胎脑cDNA文库中成功扩增出hFKBP5 2基因 ,在 pET2 8a (+ )中实现了高效、可溶性的融合表达 ,表达量约 30 % .重组的蛋白质经亲和纯化至电泳纯 ,纯化后的hFKBP5 2显示出肽基脯氨基顺反异构酶活性 .表明原核表达的hFKBP5 2具有类似于其天然蛋白质的生物学活性 .
To obtain active hFKBP52 protein for screening novel neurotrophic drugs. Semi-nested and overlap PCR and affinity chromatography were used. hFKBP52 gene was cloned successfully from human fetal brain cDNA library, and then highly expressed (about 30%) as fusion protein in pET28a (+) vector system. The recombinant protein was purified as one band on SDS-PAGE. The purified hFKBP52 showed peptidyl-prolyl cis-trans isomerase (PPIase) activity, similar to the wild type.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第2期236-239,共4页
Progress In Biochemistry and Biophysics
基金
国家"973"创新药物基金!资助项目 (G19980 5 110 7)&&
