摘要
利用PCR技术从油菜BrassicanapusH1 65基因组DNA中分离了napinB启动子。序列分析表明 ,扩增片段 (nap30 0 )与文献报道的napinB启动子相应区域的同源性为 97%。将其与gus连接构建种子特异性表达载体 ,农杆菌介导转化烟草。PCR、Southern结果显示 ,nap30 0已整合到烟草基因组DNA中 ,获得了转基因植株。
A fragment of 300bp (nap300) in .napinB. promter region was isolated by PCR from genomic DNA of .Brassica napus. H165. Nap300 and the corresponding sequence of .napinB. promoter shared 97% homology. Expression vector harboring the promoter fused with GUS gene was constructed for functional analysis. Tobacco plants were transformed by .Agrobacterium tumefaciens._mediated method. PCR and Southern results showed that nap300 had been integrated into genomic DNA of tobacco successfully. Assay of GUS activity in the seeds of transgenic tobacco will be carried out to identify the function of the promoter.
出处
《植物学通报》
CSCD
北大核心
2001年第2期216-220,共5页
Chinese Bulletin of Botany
关键词
种子
特异性启动子
植物
表达载体
转基因植株
Seed_specific promoter, Plant expression vector, GUS, Transgene, Tobacco
