摘要
目的:确定新免疫球蛋白基因SNC73在大肠癌中的组织学定位;观察它在大肠癌发生、发展不同阶段的表达状况;探讨其表达与大肠癌表型的相关性。方法:用地高辛标记的原位杂交技术检测SNC73基因在29例大肠癌病人的正常肠粘膜、癌旁粘膜、腺瘤5例以及癌组织中的表达状况。结果:SNC73基因的表达发生在大肠的正常腺上皮结构上,着色颗粒位于胞浆,胞核无或着色较淡;癌组织中没有或有少量表达,且一般较正常粘膜染色为淡;SNC73基因在肠粘膜的淋巴滤泡中可见较多的阳性染色,而在其他间质和肌层中未见明显阳性信号。正常粘膜标本中SNC基因阳性率为62.07%;癌旁粘膜中31.03%;腺瘤中20.00%;癌组织中20.69%。SNC73基因在同一大肠癌病人正常粘膜和癌组织中表达之间存在显著性差异(P<0.01)在大肠癌组织中表达的阳性率明显低于正常肠粘膜。结论:SNC73基因在大肠癌中的表达率一定程度上低于相应的正常粘膜,其生物学功能可能不同于一般意义上的免疫球蛋白。
Objectives:This study was designed to observe th e histological localization of SNC73gene expression in colorectal cancer,and to investigate their expression in different phases from normal epithelium,transit ional mucosa,adenoma to carcinoma,and to discuss the relationship between SNC73gene expression and the clinic factors of colorectal cancer.Method:With in situ hybridization,the expr ession of the SNC73was determined in29patients with colorectal cancer group specimens as the above.Results:SNC73gene expression was observed i n normal mucosal tissue and some lymph follicles of the mucosa,but se ldom in cancer tissue.The positive r ate of SNC73expression was 20.69%in colorectal cancer;was 62.07%in normal mucosa;was 31.03%in adjacent tissue;and wa s 20.00%in adenoma.Statistical difference was found between expressions in normal mucosa and those in co lorectal carcinoma from the same patient (P<0.01).Conclusions :The positive rate of SNC73expressio n in colorectal cancer is lower than t hat in corresponding normal mucosa.The biological function of S NC73may be different from that of com mon immunoglobulins.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第5期460-463,共4页
Chinese Journal of Cancer
基金
国家自然科学基金项目39170800
国家科委"863"项目(102100107)
