摘要
本文采用体外同种混合淋巴细胞实验系统观察了静息B细胞 (restingBcell)诱导的同种免疫低应答反应。C5 7BL/6小鼠脾细胞经贴壁去除巨噬细胞后 ,用抗Thy 1抗体加补体和Percoll非连续密度梯度离心法分离 ,获得纯化的静息B细胞 ,经FACS检测其表面高表达SmIg (94 88% )和处于细胞分裂的G0 期 (95 79% ) ,并表达H 2Kb(99 5 % )和H 2I Ab(82 42 % ) ,但低表达CD80 /CD86 (分别为 4 2 6 %和 4 16 % )。C5 7BL/6小鼠静息B细胞刺激同种BALB/c小鼠T细胞 (初次MLR)只呈现微弱增殖反应 ,明显低于用LPS活化B细胞或全脾细胞刺激时的反应值。作再次MLR实验时 ,经初次MLR处理的T细胞对C5 7BL/6小鼠脾细胞表现为很弱的应答反应 ,但对无关的第三品系AKR鼠脾细胞却呈现强应答 ,说明这种应答低下具有相对特异性。上述结果提示 ,静息B细胞在MLR系统中不能诱导同种T细胞的充分活化而表现为同种免疫应答的低下。这在同种移植中诱导受体对移植物耐受提供有益的启示。
In this study,the MLR assay was used to examine the capacity of resting B cells to induce allo hyporesponsiveness in vitro Resting B cells were separated from macrophage depleted splenocytes of C57BL/6 mice(H 2 b) by using anti Thy 1 antibody plus complement and discontinous dense gradients centrifugation in Percoll (95 79% of the harvested cells are at G 0 stage of the cell cycle The expression of cell surface molecules:SmIg 94 88%,H 2K b 99 50%,H 2I A b 82 44%,CD80 4 26%,CD86 4 12%). In the primary MLR the proliferation response of BALB/c T cells was much lower than that of unfractionated splenocytes or LPS activated B cells from C57 mice In the secondary MLR,the BALB/c T cells primed with resting B cells in primary MLR also showed a very low proliferation response to C57 spleen cells,but a strong proliferation response to the spleen cells from a third strain AKR mice These results indicate that T lymphocytes can not be fully activated by allo resting B cells in the MLR system and show low proliferation capacity
出处
《上海免疫学杂志》
CSCD
北大核心
2001年第2期80-82,共3页
Shanghai Journal of Immunology
基金
国家自然科学基金资助项目! (3 983 0 3 4 0 )
