摘要
目的 研究抗放药物E838对辐射诱导哺乳动物细胞恶性转化的影响。方法 用不同剂量的13 7Csγ射线照射培养的CHO和CHL细胞 ,比较在有或没有E838的情况下 ,其对细胞集落形成率以及转化率的影响。结果 在 3Gy照射剂量下 ,CHO细胞加药 1μg/ml处理组集落形成率显著高于不加药组 (P <0 0 5 ) ,而转化率加药组比不加药组低且有统计学意义 (P <0 0 1)。CHL细胞在此范围内变化亦有同样的趋势 ,但结果无统计学意义 (P >0 0 5 )。结论 E838在一定浓度范围内对辐射诱导哺乳动物细胞转化有一定的抑制作用。
Objective\ To study the effects of an anti\|radiation medicine E838 on malignant transformation of mammalian cell induced by radiation. Methods\ CHO and CHL cells were cultured in the RPMI 1640 medium with or without E838 and were irradiated with different doses of 137 Cs γ rays.The total number of colonies and type Ⅰ,Ⅱ and Ⅲ malignant transformation foci were counted for each group three weeks after irradiation. Results\ Statistic analysis indicated that the colony\|forming rate of CHO cells in the groups cultured with E838(1 μg/ml) was significantly higher than that of groups without E838 after irradiation with 3 Gy 137 Cs γ\|rays( P <0 05).The transformation rate of the group with E838 was lower than that of the group without( P <0 01).There was a similar tendency in CHL cells at the same irradiation dose,without statistical significance. Conclusion\ In the proper concentration range E838 shows certain inhibitory effects on malignant transformation of mammalian cells induced by radiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2001年第1期21-23,共3页
Chinese Journal of Radiological Medicine and Protection
基金
中国医学科学院中国协和医科大学重点基金!资助项目 (980 4)
