摘要
Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.
Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2001年第4期598-600,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金! (批准号 :2 963 2 0 0 4)资助
