摘要
目的 研究类风湿关节炎 (RA)相关的HLA DRβ1基因亚型对细胞内蛋白激酶A(PKA)信号通路的影响。方法 测定HLA DRβ1 0 40 1、 0 40 2、 0 40 3、 0 40 4亚型转基因细胞内PKA、cAMP及腺苷环化酶 (AC)的浓度及活性。结果 RA相关的HLA DRβ1 0 40 1和 0 40 4转基因细胞的PKA活力、cAMP水平及AC活力明显低于HLA DRβ1 0 40 2和 0 40 3转基因细胞 (P值均 <0 .0 1)。结论 RA共同表位QK/RRAA的表达可抑制PKA、cAMP和AC的活性 。
Objective To investigate the impact of rheumatoid arthritis (RA) associated HLA DRβ 1 *0401, *0402, *0403 and *0404 subtypes on protein kinase A (PKA) signaling. Methods To detect the activities of adenylate cyclase (AC), cAMP and PKA in transfectants expressing rheumatoid arthritis associated HLA DRβ 1 subtypes and their mutants. Results The levels of AC, cAMP and PKA of HLA DRβ 1 *0401 and *0404 transfectants were significant lower than that of HLA DRβ 1 *0402 and *0403 transfectants ( P <0.01). The mutant HLA DRβ 1 *0403 tranfectants expressing DRRAE or QRRAA secreted lower levels of PKA, cAMP and AC ( P <0.01). Conclusion RA associated HLA DRβ 1 *0401 and *0404 expression suppressed intracelluar PKA signaling pathway, and may mediate abnormal intracellular signaling in rheumatoid arthritis.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2001年第1期19-21,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金!(39970 697)
中华医学专项人才基金部分资助
