摘要
目的 :建立pcDI CCR4稳定转染并具有CCR4功能性表达的细胞株 ,为深入研究CCR4配体提供基础。方法 :利用RT PCR技术进行人CCR4的cDNA克隆化。通过基因转染技术获得稳定转染细胞株 ,利用趋化实验、钙流实验证实稳定转染细胞株能有效表达CCR4并具有生物功能。结果 :成功地将CCR4cDNA克隆于真核表达质粒pcDI,转染HEK2 93细胞获得稳定表达CCR4的HEK2 93细胞 ,可被RANTES诱导产生趋化反应和钙内流反应。结论 :建立的pcDI
Objective:To constructed a functional cell line stably transfected with human CCR4 cDNA and to study the interaction of CCR4 and its ligand Methods:RT PCR, cDNA cloning and transfecting were used for the constructing of the functional cell line stably expressing human CCR4 The chemotaxis and calcium mobilization assay were used for examining bioactivities of the cells stably transfected with CCR4 Results:CCR4 cDNA was successfully cloned into the eukaryotic vector pcDI and pcDI CCR4 was stably transfected into HEK293 cells, which can be induced a chemotactic responses and the calcium mobilization by RANTES Conclusion:We have constructed a functional cell line stably expressing human CCR4
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第3期123-124,129,共3页
Chinese Journal of Immunology
