摘要
目的 探讨利用人鼻中隔软骨细胞组织工程方法构建预定形态软骨的可能性。方法 将人鼻中隔软骨细胞播种在聚乙醇酸 (polyglycolicacid ,PGA)无纺网支架材料上 ,制成片状和管状结构 ,埋入裸鼠体内 ,经 4、6、8周后取材作大体及组织学观察。结果 大体观察见裸鼠体内形成了预定的片状和管状软骨。组织学观察 :6周时软骨细胞基本成熟 ,Masson三色染色显示胶原形成 ,番红花 “O”染色证实其基质中存在糖氨多糖。对照组于 6周时PGA纤维基本消失。
Objective To investigate the feasibility of fabricating a new cartilage in predetermined shapes with tissue engineering methods. Methods Human nasoseptal chondrocytes were seeded onto a nonwoven mesh of polyglycolic acid(PGA) to form a cell PGA construct. The construction was then configured in sheet and tube shapes, and implanted subcutaneously into the dorsa of 11 athymic mice. The specimens were harvested 4,6,8 weeks after implantation and subjected to gross morphologic and histologic analysis. Results Gross observation showed that the predetermined sheet and tube shapes of new cartilage were formed. Histological observation demonstrated that new mature cartilages were formed in 6 week. A Masson's trichrome stain showed the interwining bands of collagen at the periphery of the cartilage. Staining of Safranin O evaluated that the new cartilage was bound of glycosaminoglycan. In the control group, the PGA of the specimens were completely absorbed at 6 weeks. Conclusion Human nasoseptal chondrocytes PGA construct could develop into a new cartilage in predetermined shapes in athymic mice.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2001年第1期22-24,T005,共4页
Chinese Journal of Otorhinolaryngology
