摘要
将携带ＭＸ启动子调控的Ｃｒｅ基因真核表达载体ｐＭｘ－ｃｒｅ线性化后，通过受精卵显微注射途径制备转基因小鼠。共注射９９个卵，产仔２０只，利用ＰＣＲ对小鼠进行筛选，以基因组Ｓｏｕｔｈｅｒｎ ｂｌｏｔ确证，最后得到一个阳性的小鼠品系，进而将其保种和扩大繁殖。
To generate the transgenic mice expressing cyclization recombination enzyme, the recombinant gene, in which the coding region of cre gene is drived by the promoter of mouse Mx gene, was microinjected into pronuclei of fertilized mouse eggs. Founders of transgenic mice harbouring the recombinant gene were screened by polymerase chain reaction (PCR) at genomic DNA level and confirmed by Southern blot. One line of Mx-cre transgenic mice was obtained. Then, the Mx-cre transgenic mouse line was cultured and propagated.
基金
国家863计划项目!(102-10-04-03)
国家自然科学基金重点项目!(399993420)
国家人类基因组南方中心!(
