摘要
目的了解嗜酸性粒细胞在激素抵抗型哮喘 (SRA)的发病机理和诊断中的意义。方法采集 SRA患者和非激素抵抗型哮喘 (NSRA)患者的外周血， Percoll密度梯度法分离嗜酸性粒细胞，分别应用 DPH染色荧光分光光度计测量嗜酸性粒细膜粘性和罗丹明 123染色流式细胞仪测定其线粒体膜电位。结果 SRA患者低密度嗜酸性粒细胞 (HE)比例增加，嗜酸性粒细胞的膜粘滞度增加，线粒体膜电位的相对荧光强度下降。结论功能活跃的 HE增多可能是 SRA发生的机理之一，嗜酸性粒细胞膜流动性及线体膜电位的改变，有助于 SRA和其他哮喘的鉴别。
Objective To investigate whether eosinophil plays a role in the pathogenesis of steroie resistant astma .Method Blood specimens from SRA and NSRA patients were collected ,eosinophils were separated by Percoll's liquid ,The fluidity of DPH- stained eosinophil membrane was measured by spectrofluorometer eosinophi;'s mitochodrial membrane potential indicated by Rodamme 123was determined by flow cytometer .Result The ratio of hypodense eosinophil in SRA patients was higher than those in NSRA patients [(17.5± 3.70)]％ /(11.5± 3.03)％， P=0.0016.],The fluorescence laberled mitochondrial membrane potentials in SRA were decreased in contrast to those in NSRA[(39.35± 12.43/(57.35± 15.22),P=0.021]. Conclusion Our data suggest that active hypodense eosinophil might contribute to the pathogenesis of SRA,The esosinophil fluidity of membrane and mitochondrial membrane potential maybe used as.
出处
《现代康复》
CSCD
2001年第3期94-95,共2页
Modern Rehabilitation
关键词
哮喘
激素抵抗型
嗜酸性粒细胞
膜流动性
线粒体膜电位
asthma
steroid resistant
eosinophil fluidity of memebrane
mitochondria
membrane potential
