摘要
探讨维甲酸 ( RA)对二乙基亚硝胺 ( DENA)诱发大鼠肝癌发病过程的作用。从第 1 1周开始 ,对 DENA诱癌的 Wistar大鼠隔日腹腔注射 RA,每只动物连续给药 1 0次 ,并设对照组。在诱癌满 1 6周时 ,每组处死 7只 ,观察比较各组大鼠肝脏形成的≥ 3 mm和≥ 5mm的结节数和所见最大结节的体积。给药结束后不加其他措施直至死亡 ,计算生存时间。在给药前、结束时和给药后 2 0 d分别记录大鼠体重。实验发现 ,治疗组肝切面结节数显著小于对照组 ( P<0 .0 5) ,结节体积也显著小于对照组 ( P<0 .0 5)。与对照组相比 ,治疗组的生存时间延长 55.8% ( P<0 .0 5) ,但体重差异不明显。结果提示 ,RA可明显减轻DENA诱发大鼠肝硬化的程度、形成肝癌结节的数目和大小 ,并延缓其发展 。
To investigate the suppressive effects of retinoic acid (RA) on the hepatocarcinoma induced by N diethylnitroamine (DENA) in rats. RA(1.5 mg/kg, treated group) and the saline without RA (control group) were administrated (i.p) every other day to the rats of hepatocarcinogenesis induced by DENA beginning from the 11th week. At the beginning of the 16th week, 7 rats in each group were killed. The nodules≥3 mm and ≥5 mm in diameter and the volume of the largest nodule were calculated and compared among groups. The survival time was recorded and the rats were weighted before and after the administration. The number of the nodules ≥3 mm and ≥5 mm in treated group were significantly less than those in control group ( P <0.05). The mean volume of the largest nodule in treated group was significantly smaller than in control group ( P <0.05). Compared with control group, the survival time in treated group prolonged by 55.8%, but the bodyweights of rats in treated group did not changed. The results indicated that RA suppressed the development of primary hepatic nodules induced by DENA and prolonged the survival time of the rats.
出处
《上海实验动物科学》
2001年第1期36-38,共3页
Shanghai Laboratory Animal Science
