摘要
目的 证实在非甲～庚型病毒性肝炎患者肝组织中TT病毒 (transfusion transmittedvirus,TTV)的存在。方法 采用地高辛素标记TTVDNA探针以原位杂交技术对 5 1例血清学病毒标记非甲～戊型、免疫组化检测肝组织中HBsAg、HCVNS3Ag及HGVNS5Ag阴性的病毒性肝炎患者石蜡包埋肝组织进行了检测。结果 各型病毒性肝炎肝组织中TTV基因的总检出率为 2 7 5 % ,其中急性轻型肝炎的检出率为 30 8% (4 / 13) ,急性重型肝炎为 1/ 8,亚急性重型肝炎为 3/ 7,慢性肝炎为 2 / 6 ,活动性肝硬化为 2 / 9,慢性重型肝炎为 1/ 4,原发性肝癌为 1/ 4。TTVDNA表达于肝细胞核或胞质内 ,以核型多见。在急性肝炎 ,TTV阳性细胞弥漫分布于肝小叶内 ,慢性肝炎于汇管区附近较为密集 ,而在肝硬化病例 ,阳性细胞在假小叶内多呈片簇状不规则分布。结论 在不明原因病毒性肝炎患者血清及肝组织中TTVDNA的检出表明TTV为一种新型的肝炎病毒 。
Objective To demonstrate the existence of TT virus(transfusion\|transmitted virus,TTV) in liver tissues of patients with unknown etiology.Methods Paraffin\|embedded liver tissues from 51 cases who were non A\|E hepatitis serologically and immunohistochemically negative for HBsAg,HCV NS3 antigen and HGV NS5 antigen were tested by \%in situ\% hybridization with Dig TTV DNA probe.Results The total positive rate of TTV DNA was 27\^5%(14/51).Among the different pathological types,the positive rates were 30\^8%(4/13) in acute mild hepatitis,12.5%(1/8) in acute fulminante hepatitis,42\^9%(3/7) in subacute fulminante hepatitis,33\^3%(2/6) in chronic hepatitis,22\^2%(2/9) in active liver cirrhosis,25%(1/4) in chronic fulminante hepatitis and 25%(1/4) in primary liver carcinoma respectively.Hybridization signals were detected within the nuclei or cytoplasm of the hepatocyte and most of them in nuclei.The positive cells were diffusely scattered in the intralobular areas in acute hepatitis and aggregated in periportal areas in chronic hepatitis or in the form of clustering in pseudolobules in active liver cirrhosis. Conclusion Our results suggest that the TTV detected from liver tissues or sera from patients with unknown etiology is a novel virus,which belongs to hepadnavirus.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2001年第1期9-11,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金!(39870 6 95 )
北京自然科学基金!(7992 0 2 3)
关键词
病毒性肝炎
肝炎病毒
TT病毒
原位杂交
Hepatitis,viral
Hepatitis virus
TTV
\%In situ\% hybridization
