摘要
目的 复制可靠的脑 心综合征动物模型。方法 采用胶原酶和肝素联合注入尾状核方法建立大鼠脑出血模型 ,动态监测大鼠脑出血过程中心电图、心肌酶谱的变化 ,同时HE染色及电镜观察心肌形态学改变。结果 大鼠脑内血肿开始形成于 6h ,并于 2 4h达高峰 ,随后缩小或稳定。动物神经系统体征的出现与血肿大小的变化相一致。脑出血后心电图异常 ,血清肌酸磷酸激酶B型 (CK MB)改变及心脏损害均以血肿高峰期最为显著。结论 采用胶原酶加肝素联合进行大鼠尾状核注射的方法 ,可建立稳定、可靠、易于复制的脑出血模型。其血肿大小的变化规律比较接近临床脑出血的发生、发展过程。同时伴有心电图、血清CK MB及心肌病理形态学改变 ,说明该模型具备研究脑 心综合征的客观条件。
Objective To establish the reliable experimental animal model of cerebrocardiac syndrome(CCS).Methods The cerebral hemorrhage (ICH) was induced by infusing collagenase and heparin into rat caudate nucleus.The changes of serum CK MB and ECG were observed and the morphologic changes of myocardium were observed with light and electron microscopes.Results The intracerebral hematomas formed gradually at 6 h and peaked at 24 h,then reduced or became steady.The appearance of the syndrome was in accord with the development of hematoma.ECG abnormality,serum CK MB change and heart damage became significant during the peak time of intracerebral hematoma formation.Conclusions Infusing collagenase and heparin into rat caudate is a convenient and effective method to establish a stable,reliable and duplicable cerebral hemorrhage rat model.The changes in size of hematoma is approximatly parallel to the occcurrence and development of clinical hemorrhage.At the same time,there are ECG,serum CK MB and pathological changes,indicating that this model possesses the objective conditions for study on cerebrocardiac syndrome.
出处
《中华老年心脑血管病杂志》
CAS
2001年第2期119-121,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
