摘要
在淡色库蚊越冬过程中,测出其血淋巴含18～19种游离氨基酸。越冬期淡色库蚊除胱氨酸和脯氨酸浓度(0.2067±0.0058nmol/μl、36.5467±0.4654nmol/μl)高于越冬前期(0.1563±0.0021nmol/μl、22.2467±1.3537nmol/μl)外,其余各氨基酸及总氨基酸均显著下降;越冬后期与越冬期相比,各氨基酸及总氨基酸均显著升高,其中天门冬氨酸(0.4300±0.0520nmol/μl)、谷氨酸(3.9600±0.0794nmol/μl)、丙氨酸(22.9067±1.2860nmol/μl)、胱氨酸(1.0167±0.0551nmol/μl)和脯氨酸(47.9267±2.5597nmol/μl)及总氨基酸(98.2402±3.6139nmol/μl)甚至超过越冬前期的水平(分别为0.1900±0.0557、3.3067±0.1850、6.8833±0.1250、0.1563±0.0021、22.2467±1.3537和59.9617±1.5296nmol/μl)。用于采集血淋巴的蚊虫,胃血均为Sella 1期,卵巢发育不超过Christophers IIb期,可排除血食及卵巢后期发育对氨基酸测定结果的影响。
An analysis of the amino acids present in the hemolymph of Culex pipiens pallens caught from resting or overwintering places (blood meal: Sella 1, i.e. empty; ovary stage: under Christophers IIb) in Zhengzhou (34°43'N,113°39E) indicated that 19,18 and 18 kinds of free amino acids (FAAs) were detected during stages of preoverwintering,overwintering and pos-toverwintering respectively. Comparison of hemolymph amino acids between overwintering and preoverwintering stages revealed that the concentrations of the total FAAs and FAAs reduced significantly in overwintering except cystine and proline. The concentrations of cystine and proline during preoverwintering were 0. 1563 ±0. 0021 nmol/μl and 22. 2467 ±1. 3537 nmol/μl, but 0. 2067±0. 0058 nmol/μl and 36. 5467 ±0. 4654 nmol//μl during overwintering respectively. Comparison of hemolymph amino acids between postoverwintering and overwintering stages showed that the concentrations of all FAAs and total FAAs increased significantly during postoverwintering stage, and the concentrations of aspartic acid, glumatic acid, alanine,cystine, proline and total FAAs even exceeded those during preoverwintering stage respectively.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第1期17-20,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
越冬
血淋巴
游离氨基酸
蚊
Culex pipiens pollens, hibernation, hemolymph, free amino acid.
