摘要
目的 研究胸腔积液端粒酶活性在良恶性胸腔积液中的鉴别诊断价值 ,并与癌胚抗原(CEA)进行对比。方法 用聚合酶链反应 酶联免疫吸附分析法 (PCR ELISA)检测胸腔积液端粒酶活性 ,用酶免疫分析法 (EIA)检测胸腔积液CEA水平。根据最终诊断结果 ,6 5例患者分成 2组 :(1)非恶性胸腔积液组 :35例 ,(2 )恶性胸腔积液组 :30例。并将端粒酶活性检测结果与胸腔积液CEA测定结果进行比较。结果 非恶性胸腔积液中端粒酶阳性 2例 (5 7% ) ,恶性胸腔积液中阳性 2 7例 (90 % ) ,端粒酶活性测定诊断恶性胸腔积液的灵敏度为 0 90 ,特异度 0 94,阳性预测值 0 93 ,阴性预测值0 92 ,正确率 0 92。CEA诊断灵敏度为 0 6 0 ,特异度 0 89,阳性预测值 0 82 ,阴性预测值 0 72 ,正确率0 75。结论 胸腔积液端粒酶活性鉴别良恶性胸腔积液的诊断效能明显优于CEA测定 。
Objective To study the diagnostic value of telomerase activity in differentiating malignant from nonmalignant pleural effusions. Method Sixty five specimens of pleural effusion were examined for telomerase activity and CEA level by PCR ELISA and EIA,respectively According to the final diagnosis achieved,the patients were classified into two groups :group Ⅰ, 35 nonmalignant pleural effusions,group Ⅱ, 30 malignant pleural effusions diagnosed by either fluid cytology or pleural biopsy The results of telomerase activity were compared with CEA levels. Results Two of thirty five(5 7%) samples of pleural effusions in group I were found to possess weak telomerase activity and 27 of 30(90%)samples in group Ⅱ showed positive enzymatic activity The diagnostic sensitivity is 0 90,specificity is 0 94,and the positive and negative predictive value are 0 93 and 0 92,respectively The overall accuracy is 0 92 The diagnostic efficiency of CEA as following: sensitivity 0 60,specificity 0 89,positive and negative predictive value are 0 82 and 0 72,respectively,accuracy 0 75. Conclusion The results indicate that the detection of telomerase activity in pleural effusions is more sensitive,more accurate than CEA in differentiating benign from malignant effusions and can be used as an adjunctive measure in the diagnosis of malignant pleural effusions
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2001年第4期201-203,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
