摘要
本文应用显微组织化学鉴定的方法,氯化三苯四氮唑(TTC)法,对寄生植物肉苁蓉种子的活力进行测定并对TTC法的测定方法和测定条件进行了研究。同时在大田进行了肉苁蓉种子播种试验。研究结果表明;寄生植物肉苁蓉种子的活力可采用组织化学的方法进行测定,细胞色素氧化酶、过氧化物酶、三磷酸腺苷酶的活性随种子年代的加长呈明显下降趋势,与TTC法测定结果一致。用TTC法测定寄生植物肉苁蓉种子活力的适宜测定条件是:用pH7.0磷酸缓冲液37℃、浸泡种子8h.然后在0.5％TTC溶液中、23℃、自然光照下染色16h。
In this paper we reported the study on determination of seed vigor of Cistanche deserticola Ma by the Cyto-chemical methods and TTC-test. At the same time, the field planting trials of Cistanche de- serticola seeds have been carried out in the farm land. The results indicate that seed vigor of Cistanche deserticola may be tested by the histo-chemical method. The activities of cytochrome oxidase, peroxi- dase and adenosine triphosphatase obviously decreased as the time of storage was prolenged. These re- suits Coincide with the results of TTC-test. The optimum conditions of TTC-test on Cistanche deserti- cola seed vigor are soaking the seeds in pH 7. 0 PB. at 37℃, for 8 hours, then tramsfering into 0.5% TTC, at 23℃, staining for 16 hours under daylight.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1993年第1期95-103,共9页
Journal of Inner Mongolia University：Natural Science Edition
关键词
种子活力
肉苁蓉
寄生植物
seed vigor
Cistanche deserticola
parasitic plant
TTC-test
