摘要
目的研究酶校准品用于校准不同的“仪器 /试剂”测定系统时 ,能否增加各系统间血清酶测定结果的可比性。方法在日立 7170 A和 CX9全自动生化分析仪上用 4种不同的试剂分别测定 15 0份不同浓度分布的新鲜血清中的丙氨酸转氨酶 (AL T) ,天门冬氨酸转氨酶 (AST) ,碱性磷酸酶〔(AL P) ,10 0份〕,肌酸激酶 (CK) ,γ-谷氨酰转肽酶 (GGT) ,乳酸脱氢酶 (L D)等 6种酶的活性。同 1份标本用 4种不同的“仪器 /试剂”测定系统进行校准前和校准后测定 ,比较各测定系统测定前后的均值。结果发现不同的“仪器 /试剂”系统间的测定结果存在一定差异 (各系统间均值的 CV值≥ 7.0 % ,其中 AL P为 2 7.0 % ,GGT为 17.4% ) ,用统一的酶校准品校准后 ,各系统间测定结果的差异明显减小 (除 AL P和 GGT的 CV值分别为 6 .6 %和 5 .1%外 ,其余各项目的 CV值均 <5 % )。结论统一的某些酶校准品能增加不同的测定系统间结果的可比性。
Objective To study the effects of calibrators in unifying the results of different analyzer/reagent systems. Methods The activities of ALT, AST, CK,GGT, LD in 150 and ALP in 100 fresh sera were measured by using four analyzer/reagent systems, and the mean values of different systems were compared between, before and after calibration with calibrators. ResultsThe differences among the results of four systems were observed before calibration. The coefficients of variation(CVs) are all more than or cqual to 7.0%,more remarkably these of ALP and GGT are 27.0% and 174% respectively. After calibration, the differences are decreased clearly and the CVs are all lower than 5% except that ALP and GGT are 66% and 51% respectively. ConclusionEnzyme calibrators could be useful in standardizing the results of different analyzer/reagent systems.
出处
《上海医学检验杂志》
北大核心
2001年第2期79-80,83,共3页
Shanghai Journal of Medical Laboratory Sciences
