摘要
目的:研究结核性胸膜炎与恶性胸水患者胸腔积液单个核细胞经结核分枝杆菌特异性抗原肽刺激后IL-22的表达特点,探索IL-22对结核性胸膜炎和恶性胸水的鉴别诊断价值。方法:采用eBioscience IL-22和IFN-γ试剂盒,利用流式细胞术微球阵列法检测52例结核性胸膜炎和35例恶性胸水患者胸水上清、PFMCs经结核分枝杆菌特异性抗原肽刺激培养后上清中IL-22和IFN-γ的浓度,并作统计学分析。结果:结核性胸膜炎组IL-22在刺激后上清中表达水平显著升高,且显著高于恶性胸水组(P<0.05)。结核性胸膜炎组IL-22与IFN-γ在刺激后上清中表达水平显著相关(r=0.3485,P=0.0320)。结论:结核分枝杆菌抗原特异反应性IL-22可作为鉴别诊断胸腔积液病因的新指标。
Objective： To study the characteristics of the expression of IL-22 generated by the PFMCs of TPE and MPE was stimulated by ESAT-6 and CFP-10 and to probe into the diagnose value in TPE and MPE. Methods： The eBioscience IL-22 and IFN-γ kits and the Flow cytomix streaming technology were used to test the concentration of IL-22 and IFN-γ, the PFMCs culture fluid supematant and the PFMCs culture fluid supernatant stimulated by specific MTB antigen peptides of 52 patients with TPE and 35 patients with MPE, and the results were statistically analyzed. Results： The concentrations of IL-22 was elevated significantly in PFMCs stimulated by peptides in TPE group which was significantly higher than that of the MPE group（P〈0.05）. The concentrations of IL-22 and IFN-γ in PFMCs stimulated by peptides were correlated in TPE group （r=0.3485, P=-0.0320）. Conclusion： Mtb-antigen-specific IL-22 become a the new biomarker for pleural effusions differential diagnosis.
出处
《现代生物医学进展》
CAS
2014年第20期3922-3924,3959,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81071318)
关键词
白细胞介素-22
结核性胸膜炎
恶性胸水
鉴别诊断
Interleukin-22
Tuberculous pleurisy
Malignant pleural effusions
Differential diagnosis
