摘要
目的:检测碘131(131I)对转染了人钠/碘转运体(hNIS)基因的人结肠癌细胞(SW480细胞)的杀伤作用。方法:将SW480细胞分为转染组、空白质粒转染组(空白质粒组)和空白对照组,行体外摄131I试验、高氯酸盐抑制实验,及Annexin V-FITC/PI双染行流式细胞仪检测细胞凋亡情况。结果:转染组与空白对照组及空白质粒组比较,摄131I能力均增高8倍;高氯酸盐可以有效地抑制转染组的摄131I能力;Annexin VFITC/PI双染后流式细胞仪检测结果显示,转染组凋亡指数高于空白对照组和空白质粒组。结论:131I对转染hNIS-SW480细胞具有显著杀伤效果。
Objective： To detect the killing effect of colon cancer cells （SW480） by iodine 131 （131I）. Methods： Cell apoptosis was detected when taken in131I in vitro test and perchlorate suppression experiments and AnnexinV-FITC/PI double dye lfow cytometry instrument.Results： Stably-hNIS-gene-expressing cell line was about 8 times higher than the control group and blank plasmid group in 24 h iodine uptake capacity. Per-chlorate inhibition tests showed that the iodine uptake of hNIS-SW480 was the hNIS gene’s function. AnnexinV-FITC/PI double dye lfow cytometry showed apoptosis index in the transfection group was higher than that of the control group and blank plasmid group. There were signiifcant differences that radioactive iodine SW480 cells transfected with better killing ability under 100 μCi131I in 24 h.Conclusion： 131I has signiifcant cell killing effect on SW480 cells transfected hNIS.
出处
《温州医学院学报》
2014年第5期334-337,共4页
Journal of Wenzhou Medical College
基金
温州市科技局科研基金资助项目(Y20090094)
关键词
钠
碘转运体
碘131
基因转染
结肠肿瘤
sodium/iodide symporter
131I
gene transfection
colonic neoplasms
