摘要
目的研究肾上腺髓质素(ADM)和整合素αvβ3在人骨肉瘤组织的不同临床分期、病理分级及组织学分型中的表达情况,并探讨二者之间的关系。方法应用免疫组化SABC法和图像分析技术的方法检测30例骨肉瘤组织中ADM和整合素αvβ3表达的阳性率、强度和平均光密度(ALD)。每张切片分别由两名病理医师在未知临床和病理资料的情况下独立观察切片,随机观察(至少5个具有代表性的高倍视野,计数100个细胞)判定肿瘤分期、分级及细胞染色程度。结果 ADM与整合素αvβ3表达的阳性率和表达强弱均随着骨肉瘤分期增加而上调(P<0.05),并在各期之间的差异有统计学意义(P<0.05)。ADM与整合素αvβ3的表达水平呈正相关。结论 ADM和整合素αvβ3在骨肉瘤中的表达强弱与骨肉瘤的恶性程度密切相关,且二者的表达变化呈正相关。
Objective To study the expression and relationship between adrenomedullin（ADM）and integrinαvβ3 in different pathological grades,clinical stages and histologic subtypes of human osteosarcoma. Methods The expression positiverate,intensity, the average optical density（ALD） of ADM and integrinαvβ3 in 30 osteosarcomacases was detected with immunohistochemical SABC technique and image analysis system. Each pathological section observed by two pathologists respectively in the case of unknown clinical and pathological information. At least five representative high field of vision counting 100 cells observed at random, in this way the degree of tumor grade,stage and cell staining were determined by two pathologist who unknown clinical and pathological data. Results The positive rate and intensity of ADM and integrinαvβ3 were significantly increased with the increase of osteosarco- ma stage（P〈0.05） and the difference was significant in each stage（P〈0.05）. The expression of ADM was positive correlated with the expression of integrinαvβ3. Conclusion The expression of ADM and integrinαvβ3 in different clinicopathological grades osteosar coma are positive associated with malignant degree of tumor , which suggest that both of them play an important role in malignant biological behaviors of human osteosarcoma.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第19期2428-2431,共4页
Chongqing medicine
