摘要
以人正常肝细胞HL-7702为研究对象,探讨碳酸钙材料对细胞的体外毒性.将HL-7702细胞暴露于碳酸钙质量浓度为0、5、10、20、40、60、80、100μg/mL的悬浮液中,通过CCK-8法检测暴露于碳酸钙材料悬浮液24 h后HL-7702细胞的存活率,并且检测HL-7702细胞内活性氧的含量.结果表明,该碳酸钙材料在实验剂量下对HL-7702无细胞毒性,能够引起人肝细胞内产生少量的活性氧(ROS).这些结果说明碳酸钙材料在一定剂量下对HL-7702细胞具有良好的体外细胞亲和性(毒性为0级或1级).
The aim of this study is to explore the cytotoxicity of CaCO3 particles on the human hepatocyte HL -7702 in vitro. HL- 7702 ceils have been treated with CaCO3 at the final concentrations of 5,10,20,40,60, 80,100μg/mL and saline has been used as control. CCK -8 assay which has been conducted to detect the effects on the survival rate of the cells exposed to CaCO3 for 24 h. The concentrations of reactive oxygen species （ROS） of HL-7702 cells have been determined. The results show that CaCO3 particles have no cytotoxicity and can induce the low levels of intracellular reactive oxygen species （ROS）. These indicate that calcium carbonates particles are nontoxic in vitro cellular compatibility with their toxicity reaction ranking level 0 or 1.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期546-550,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(30901221)
天津市应用基础及前沿技术研究计划项目(10JCZDJC17100)
