摘要
探究乌梁素海富营养化湖泊湿地演化过程中,不同植物群落下土壤氨氧化细菌群落的组成、丰度、亲缘关系以及多样性变化,结合湿地理化因子探讨基质条件对氨氧化细菌群落结构的影响。提取沉积物和土壤总DNA,对氨氧化细菌群落的amoA基因构建克隆文库,并进行测序,分析湿地沉积物与土壤过渡带4个样点中氨氧化细菌群落结构的组成、丰度、亲缘关系以及多样性指标;分析基质条件变化对氨氧化细菌群落结构的影响。富营养化湖泊湿地水陆过渡带的芦苇沼泽沉积物、碱蓬盐碱化土壤和白刺荒漠化土壤中,氨氧化细菌群落结构组成相似性逐渐降低;优势种群发生明显变化,氨氧化细菌从与Nitrosomonaslike序列相似为主要优势类群向与Nitrosospira-like序列相似为主要优势类群转变,群落结构空间异质性成因主要由总氮和水溶盐总量这两个基质因子所主导,相关系数r为0.943;多样性指数分析表明,芦苇沼泽沉积物和白刺荒漠化土壤适合多样的氨氧化细菌生长。氨氧化细菌多样性与优势种群在湿地水陆过渡带发生明显变化,氨氧化细菌群落结构主要驱动因子为总氮和水溶盐总量的组合。
The aim of this study is to investigate soil ammonia-oxidizing bacterial community composition,abundance,phylogenetic relationship and diversity under different plant communities formed in the evolution process of eutrophic inland lake Wuliangsuhai,and to discuss the impacts of soil physicochemical factors on ammonia-oxidizing bacterial community structure. The total DNA was extracted and a clone library was constructed based on amoA gene in order to analyze ammonia-oxidizing bacterial community composition,abundance,phylogenetic relationship and diversity index. Soil substrate conditions were analyzed to assess their effects on ammoniaoxidizing bacterial community composition. The similarity of ammonia-oxidizing bacterial community composition gradually decreased from eutrophic lake sediments to desertification soil. The dominant species significantly changed from the Nitrosomonas-like group to the Nitrosospira-like group. Correlation analysis indicated that the combination of total nitrogen and total water soluble salt had the most significant effect on the ammonia-oxidizing bacterial community structure,and the correlation coefficient was 0. 943. The diversity index showed that reed swamp and Nitraria tangutorum desert soil were more suitable for diverse ammonia-oxidizing bacteria to grow. Ammonia-oxidizing bacterial diversity and dominant species changed significantly in the transition zone between wetland sediment and desert soil. Total nitrogen and total water soluble salt are the major environmental factors influencing spatial heterogeneity of ammonia-oxidizing bacterial community composition.
出处
《生态学杂志》
CAS
CSCD
北大核心
2014年第7期1902-1910,共9页
Chinese Journal of Ecology
基金
国家自然科学基金项目(31160129)
国家重点基础研究计划前期研究项目(2009CB125909)
国家科技支撑计划项目(2011BAC02B03)资助
关键词
乌梁素海湿地
富营养化湖泊
氨氧化细菌
空间异质性
基质条件
Wuliangsuhai wetland
eutrophic lake
ammonia-oxidizing bacteria
spatial hetero-geneity
substrate condition
