摘要
利用细胞固定化制备苏云金芽胞杆菌LLP29-M19漂浮剂,为提高其漂浮效果,应用数学统计方法对该菌株漂浮剂配方进行优化.通过单因素试验确定海藻酸钠质量分数为1.0%,CaCl2质量分数为2.0%,空心玻璃微珠质量分数为3.5%;采用Plackett-Burman设计对影响该漂浮剂漂浮效果的8个因素进行筛选,确定漂浮剂配方中的3个重要影响因子为CaCl2、固定化时间和黑色素;通过最陡爬坡试验逼近以上3个因子的最大响应区域后,采用响应面分析获得这3个重要因子的最佳条件,确定LLP29-M19漂浮剂最佳配方:海藻酸钠0.80%、CaCl24.90%、空心玻璃微珠3.2%、菌粉3.0%、黑色素0.012%、苯甲酸钠0.3%、小白鼠饲料0.5%,固定化时间4.74 h.优化后的漂浮剂漂浮效果为1698.25,与响应面数学模型预测值的误差仅1.8%,比初始漂浮剂配方漂浮效果(64.5)提高了25.32倍.
In order to improve the suspended effect of the floating pesticide granules Bacillus thuringiensis LLP29-M19, the mathe-matical-statistics method was employed in this study. According to single factorial experiments, the concentrations of sodium algi-nate, CaCI2 and hollow glass bead were 1.0%, 2.0% and 3.5%, respectively. The three important factors of CaC12, immobilizedtime and melanin were found according to the Plackett-Burman experimental design. Then steepest ascent procedures were applied toapproach the optimal regions, and finally the optimal combined concentrations of the three factors were also determined by adoptingresponse surface methodology （RSM）. The results showed that the best formulation composition was as follows： 0.80% sodium algi-nate, 4.90% CaCl2, 3.2% bellow glass beads, 3.0% bacterial powder, 0.012% melanin, 0.3% sodium benzoate, 0.5% micefeed with the immobilized time 4.74 h. After optimization, its suspended effect was 1698.25, which increased 25.32 times comparedwith the preliminary formula, while the error between the actual value and the predictive value of RSM was only 1.8%.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2014年第3期235-240,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家863项目(2011AA10A203)
国家自然科学基金项目(31071745
31201574
31301724)
福建省高校杰出青年科研人才培育计划项目(JA12092)
福建省科技计划重点项目(2013N0003)
福建省自然科学基金项目(2013J01079
2011J01076)
