摘要
目的:探讨丹红注射液对缺氧性呼吸抑制的作用及其相关机制。方法:记录膈肌肌电观察缺氧后呼吸的变化,通过免疫组化的方法检测缺氧大鼠脑干酸敏感离子通道1a(ASIC1a)的表达。结果:大鼠对缺氧的呼吸反应为先兴奋后抑制。缺氧后30 min,单纯缺氧组大鼠呼吸较缺氧前表现为明显的抑制(P<0.05),而缺氧加丹红保护组大鼠呼吸依然兴奋(P<0.05),尚未表现出明显的抑制。大鼠脑干ASIC1a的表达主要在孤束核和斜方体核,缺氧后大鼠ASIC1a的表达较对照组明显增加(P<0.05),但缺氧加丹红保护组大鼠ASIC1a的表达较单纯缺氧组明显减少(P<0.05)。结论:丹红注射液能明显延迟缺氧后呼吸抑制的发生,在缺氧性呼吸抑制时保护机体。ASIC1a可能参与了这一过程。
AIM： To investigate the effect of Danhong injection on respiratory inhibition induced by hypoxia and its related mechanism .METHODS：The electromyogram of the diaphragm was monitored to observe the respiratory responses of the rats to hypoxia .The expression of acid-sensingion channel 1a （ASIC1a） in the brainstem after hypoxia was detected by the technique of immunohistochemical staining .RESULTS： The respiratory responses of the rats to hypoxia were initiatory excitation and consequent inhibition .In contrast to prehypoxia, the respiration of the rats in hypoxia group was inhibited 30 min after hypoxia （P〈0.05）, but the respiration of the rats in hypoxia plus Danhong group was still excited （P〈0.05）, indicating that the respiration of the rats in hypoxia plus Danhong group was not yet depressed at the same time.The ASIC1a positive neurons were detected in the nuclei of trapezoid body and solitary tract .Compared with control group, the expression of ASIC1a was obviously enhanced after hypoxia .In contrast to hypoxia group , the expression of ASIC1a in the rats in hypoxia plus Danhong group was remarkably reduced （P〈0.05）.CONCLUSION：Danhong injection resists the occurrence of respiratory inhibition after hypoxia , and ASIC1a may participate in this process .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第6期1123-1126,1135,共5页
Chinese Journal of Pathophysiology
关键词
丹红注射液
缺氧
呼吸抑制
酸敏感离子通道
Danhong injection
Hypoxia
Respiratory inhibition
Acid-sensing ion channels
