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新构建FlaA N/C蛋白辐射防护相关miRNA芯片谱研究

Effects of New Recombinant FlaA N/C Protein on Radioprotective Related miRNA Chip Spectrum
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摘要 目的通过miRNA芯片谱研究,分析FlaA N/C蛋白辐射防护功能的转录后调控机制。方法FlaA N/C蛋白皮下注射小鼠2h后,取小肠组织进行miRNA芯片谱分析,芯片谱结果用生物信息学方法预测miRNA作用靶点及可能相关的信号通路。结果 FlaA N/C皮下注射后2h有108个miRNA上调,59个miRNA下调,差异miRNA预测靶点主要集中在NF-κB、PI3K-Akt、p53和Caspase等信号通路。结论凋亡相关信号通路在FlaA N/C介导辐射防护机制中具有重要作用,有必要针对凋亡相关miRNAs网络开展辐射防护机制研究。 Objective To analysize the post-transcriptional regulation mechanism of FlaA N/C protein,chip spectrum of miRNA were studied. Methods Intestinal tissue were collected for miRNA chip spectrum analysis 2 hours after subcutaneously injection of new recombinant FIaA N/C protein in miee. Subsequently, bioinformatics were applied to forecast the target proteins and related signaling pathways. Results 108 miRNAs were up-regulated and 59 miRNAs down-regulated. The target proteins were mainly distributed in NF-kB,PI3K Akt,p53,and Caspase pathways. Conclusion Apoptosis related signal pathway plays an important role in the mechanisms of radioprotection. Further study should focus on apoptosis related miRNAs network.
作者 许颖 王丹 石娇 周晓萍 王建 常晓松
机构地区 成都医学院第一附属医院检验科 四川出入境检验检疫局
出处 《成都医学院学报》 CAS 2014年第3期249-252,共4页 Journal of Chengdu Medical College
基金 国家自然科学基金(NO:81001345) 四川省科技厅应用基础研究计划项目(NO:2014JY0039)
关键词 辐射防护 miRNA芯片谱 凋亡相关信号通路 Radioprotective miRNA Chip Spectrum Apoptosis Related Signal Pathway
分类号 R144 [医药卫生—公共卫生与预防医学]
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参考文献20

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成都医学院学报
2014年 第3期

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