摘要
目的观察脑动静脉畸形(arteriovenous malformation,AVM)患者外周血内皮祖细胞(endothelial progenitor cells,EPCs)数量和基质细胞衍生因子-1α(stromal cell-derived factor-l,SDF-1α)的变化。方法 15例首次接受治疗的脑AVM患者和15例健康对照者,应用密度梯度离心法分离外周血单个核细胞,以CD133+、CD34+、KDR+三阳性细胞为EPCs,应用流式细胞仪检测EPCs占外周血单个核细胞的百分比,ELISA法检测外周血SDF-1α浓度,比较两组差异;EPCs百分比、SDF-1α浓度与AVM大小,以及EPCs百分比与SDF-1α浓度分别进行相关性分析。结果 AVM患者外周血EPCs占外周血单个核细胞百分比及SDF-1α浓度高于健康对照者(t=4.051,P<0.05;t=3.606,P<0.05);EPCs百分比及SDF-1α与病灶大小不相关(r=0.092,P>0.05;r=0.027,P>0.05),EPCs百分比与SDF-1α正相关(r=0.461,P<0.05)。结论脑AVM患者存在外周血EPCs和SDF-1α水平升高的现象,可能与AVM患者骨髓EPCs动员增加,参与AVM血管重塑和血管新生有关。
Objective The purpose of this study was to investigate if circulating endothelial progenitor cells （EPCs） and stromal cell-derived factor-1α（SDF-1α） are increased in patients of cerebral arteriovenous malformation （AVM）. Methods Fifteen AVM patients not receiving any treatment and 15 healthy people were included in this study. Peripheral blood mononuclear cells were isolated by density gradient centrifugation and EPCs was characterized by triple staining using antibodies against CD133, CD34, and vascular endothelial growth factor receptor-2（KDR）. The percentage of circulating endothelial progenitor cells were analysised by flow cytometry. Serum concentrations of SDF-1α was determined by using enzyme-linked immunosorbent assay （ELISA）. Results The percentage of circulating EPCs and concentrations of SDF-1α were significantly higher in AVM group than in control group（t=4.051, P 〈 0.05 and t=3.606, P 〈 0.05, respectively）. There was a positive correlation between the percentage of circulating EPCs and concentrations of SDF-1α in AVM patients （r=0.461, P 〈0.05）. However, there were no correlation either between the percentage of circulating EPCs and the size of AVM or between concentrations of SDF-1α and the size of AVM in AVM patients （r=0.092,P 〉 0.05 and r=0.027, P 〉 0.05 respectively）. Conclusions The percentage of circulating EPCs and concentrations of SDF-1α are increased in AVM patients and the increase in the mobilization of EPCs from bone marrow may play a role in blood vessel remodeling and neovasculization in AVM patients.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2014年第5期279-283,共5页
Chinese Journal of Nervous and Mental Diseases
基金
国家自然基金面上项目(编号:81171102)
关键词
脑动静脉畸形
内皮祖细胞
SDF-1Α
Cerebral arteriovenous malformation Endothelial progenitor Cells SDF-1α
