摘要
为了对心脏囊肿状病变的西伯利亚鲟进行病理组织学观察和病因初步探讨,使用病理组织学和电镜观察技术,对患病西伯利亚鲟各器官进行组织病理学观察。结果表明,患病西伯利亚鲟心脏表面可观察到米粒大、息肉状、紫褐色的囊状肿物,似桑葚状,心外膜上皮细胞和结缔组织明显增生,形成囊肿。组织病变为心外膜囊肿,呈动态发展过程,主要表现为囊肿上皮由扁平变为立方上皮;囊肿壁逐渐增厚与分化,使囊腔不断增多变大,腔内有大量未分化的圆球形、梭形等不同成熟度的血细胞,形成血管组织,并浸润着大量游离或团状分布细胞,整个病变类似一种肿瘤型的恶性增生,心肌细胞肿胀,断裂,溶解,坏死;肝细胞脂肪变性并浸润炎性细胞;肾间质和脾脏淋巴细胞增多。鳃小片之间观察到大量细菌侵袭上皮细胞;鳃小片上皮细胞严重肿胀增生。电子显微镜观察到心脏、肝脏、脾脏和肾脏中有大量细菌;该病可能与微量元素有关,所观察到的细菌不是直接病因。本实验对西伯利亚鲟桑葚心病的组织病理变化进行了较为系统的研究,为进一步查明病因、临床诊断防治和致病机理等相关研究提供基础资料。
In recent years,a kind of disease broke out in Siberian sturgeon breeding base of Beijing. The main symptoms of diseased Siberian sturgeon were that the heart surface was observed to have a large number of rice grain-like,purple-brown cystic neoplasm. The whole heart looks like mulberries shape. The mortality rate were as high as 60% or more. Sick fish were about one year old. Water temperature was around 27 ℃ or so. In order to diagnose and preliminarily discuss the cause on heart surface cystic neoplasm of Siberian sturgeon,the histopathologic changes of the diseased Siberian sturgeon and the morphology of the pathogen were observed under optic and electronic microscopes. The results show that the heart surface of sick Siberian sturgeon can be observed in a large of rice grain-like,purple-brown cystic neoplasm. The heart was like mulberries shape. The epicardial mesothelial cells and connective tissue were between obvious hyperplasia. Neoplasm was formed. Epicardial lesions were dynamic process. Epicardial lesions were becoming more severe from mild. The main show were epithelium by flat to cuboidal epithelium. The cyst walls were gradually thickening and differentiating,so that the increasing cysts lined become more. The cyst cavity had a number of undifferentiated spherical,spindle and so on the different mature degrees of blood cells and vascular tissue,and infiltration with a lot of free or round state distribution inflammatory cells. The whole lesions looked like a kind of tumor type of malignant hyperplasia,myocardial cells were swelling,fractured,dissolved and necrotic. The liver lesions were hepatic steatosis and infiltration of inflammatory cells. Renal interstitium and spleen lymphocytes increased. Gill lamellae were observed between the large number of bacterial invading epithelial cells; the epithelial cells of gill lamellae were severe swelling and hyperplasia. Under transmission electronic microscope,a large number of bacteria were observed in the heart,liver,spleen and kidney. The microscopic and ultrastructure changes speculate that its etiology may be associated with trace elements and the bacteria are not the direct cause of the disease. To provide basic information for the prevention and treatment,clinical diagnosis and pathogenesis and related research,the hispathological changes were more systematically researched on Siberian sturgeon mulberry heart disease.
出处
《水产学报》
CAS
CSCD
北大核心
2014年第6期904-911,共8页
Journal of Fisheries of China
基金
国家科技支撑计划(2012BAD25B00)
现代农业产业技术体系建设专项(CARS-4609)
关键词
西伯利亚鲟
桑葚心
组织病理学
Acipenser baeri
mulberry heart
histopathology
