摘要
用含1.345 mg/ml(24 h IC50)蛇床子素的改良TYI-S-33培养基培养蓝氏贾第鞭毛虫滋养体,采用扫描和透射电镜观察蛇床子素作用后24 h虫体超微结构的变化。结果显示,经蛇床子素作用后,鞭毛虫滋养体表面凹凸不平,腹吸盘和中体附近表面有明显的病变,主要表现为腹吸盘表面细胞膜破裂,细胞内容物外溢、稀疏,出现板层样结构,空泡化严重,细胞核畸形,边缘锯齿状,核内染色质边集,吸盘微管部分结构崩解。
Giardia lamblia trophozoites were cultivated axenically in TYI-S-33 modified medium containing 1.345 mg/ml of osthole （24 h IC50）. The parasites were observed by scanning and transmission electron microscopes after treated with osthole for 24 h. The surface of the trophozoites treated with osthole was rough. The surface of ventral sucker and median body had obvious lesions, the cell membrane was damaged and the content spilled out. There were a lot of vacuoles in the cytoplasro. And the nuclear was severely deformed with a serrated edge and marginated nuclear chromatin. The microtubules of sucker had partially disintegrated.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2014年第3期242-244,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金(No.30970322)
安徽科技学院校级重点项目(No.ZRC2014406)
安徽科技学院重点学科建设项目(No.AKXK20101-2)~~
