摘要
精原干细胞（spennatogonial stem cells，SSCs）是雄性动物体内能进行终生自我更新并能将亲代基因遗传给予子代的一类细胞。不同年龄段的小鼠有不同的建系方法。6-7d幼鼠，可以用差异贴壁或直接贴壁法；5-6周成年鼠，一般采用差异贴壁法；31周老年鼠，最好种于饲养层细胞上。通过对精原干细胞系的甲基化和特异基因分析以及睾丸体内移植验证分析，成功建立了具有功能的不同年龄段的小鼠精原干细胞系。
Spermatogonial stem cells （SSCs） are a pool of cells which are capable of self-renewal and differentiation in male animals＇ whole-life. We established several SSC lines of mice at different ages using three methods. The results showed that two methods （difference adherence and direct adherence） could be used to establish SSCs in the 6-7 d little mice, but the SSCs from 5-6 weeks＇ mice were established only by the difference adherence. However, we did not get the SSCs lines of 31 weeks mice using the methods which were mentioned above other than culture on feeder cells. Finally, the functions of those SSCs were determined through analysis of spermatogonial stem cell lines methylation, specific genes expression and in vivo testis transplantation validation.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2014年第6期752-757,共6页
Chinese Journal of Cell Biology
基金
四川省教育厅重大培育项目(批准号:13CZ0029)
三峡库区生态环境与生物资源省部共建重点实验室开放课题基金项目(批准号:SKL-2011-05)资助的课题~~
关键词
精原干细胞
睾丸
体外培养
spermatogonial stem ceils （SSCs）
testis
in vitro culture
