摘要
目的比较快速尿素酶法、HE染色、亚甲蓝特殊染色以及免疫组化染色法在诊断胃黏膜病变中幽门螺杆菌(helicobacter pylori,HP)感染的价值。方法收集214例行胃镜检查并行活检的病例,采用快速尿素酶法、HE染色、亚甲蓝特殊染色及免疫组化染色法分别检测胃黏膜活检组织中HP的感染状况。结果 4种方法检测胃黏膜活检组织中HP的感染率差异具有显著性,HP阳性率从低到高依次是HE染色(27.6%,59/214)、快速尿素酶法(30.8%,66/214)、免疫组化染色(54.2%,116/214)和亚甲蓝染色(57.0%,122/214),亚甲蓝染色HP检出率最高,但存在一定的假阳性(假阳性率为34.7%,34/98)。结论免疫组化染色法检测HP清楚直观,结果准确可靠,是检测胃黏膜活检标本中HP感染的最佳方法。
Purpose To investigate the value of rapid urease test, HE staining, methylene blue staining and immunohistochemical staining in detecting Helicobacter pylori （HP） infection from gastric mucosa biopsy. Methods 214 cases of gastric mucosa specimens were collected by endoscopic biopsy and HP infection states of all samples were tested by four different methods, rapid urease test, HE staining, methylene blue staining and immunohistochemical staining. Results The rates of HP infection detected by four methods had significant differences. The positive rate of HP infection by HE staining was 27. 6% （59/214）, followed by rapid urease test 30. 8% （66/214）, immunohistoehemistry staining 54. 2% （ 116/214 ） and methylene blue staining 57. 0% （ 122/214 ）, respectively staining and rapid urease test were rapid, easy but insensitive in detecting HP infection in gastric biopsy. Methylene blue staining was sensitive in detecting HP from gastric mucosa, but the high rate of false-positive （34. 7%, 34/98） indicated it was not a reliable method. Conclusion Immunohistochemical staining of HP with the specific antibody is easy to observe, clear and accurate. It is reliable and the best method in detecting HP infection of gastric mucosa biopsy.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2014年第6期622-624,628,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(81160252)
宁夏自然科学基金(NZ11192)
关键词
胃黏膜
活检
幽门螺杆菌
方法
gastric mucosa
biopsy
helicobacter pylori
method
