摘要
骨髓间充质干细胞(BMSCs)作为一种具有多向分化潜能的干细胞广泛地运用于组织再生工程中。在促BMSCs向心肌样细胞分化的过程中,我们探讨周期性双轴力学应变对其的影响。本实验分为三组:力学应变组,将大鼠BMSCs(rBMSCs)接种于硅胶膜上,施加应变大小10%、频率1Hz力学应变,持续时间分别为6、12、24h;空白对照组,将rBMSCs接种于硅胶膜上,用完全培养基培养;基底对照组,将rBMSCs接种于六孔板中,用完全培养基培养。两组对照组分别于力学应变组加力相同时间点收集样本。通过实时荧光定量PCR方法检测各组rBMSCs的GATA4和肌细胞特异性增强因子-2C(MEF-2C)的mRNA表达,并利用Western blot方法检测各组rBMSCs的缝隙连接蛋白43(Cx43)的表达。本实验结果表明,不同持续加力时间的力学应变组的GATA4、MEF-2C的mRNA表达量以及Cx43的蛋白表达量比相应的空白对照组明显增高(P<0.05)。本研究提示,体外周期性双轴力学应变可以促进rBMSCs向心肌样细胞分化,但其具体机制尚未明确。
Bone marrow-derived mesenchymal stem cells （BMSCs） are multipotent stem cells that differentiate into a variety of cell types and widely used in tissue regeneration engineering. The purpose of this study is to investigate whether the cyclic biaxial stretching strain could promote the rat BMSCs （rBMSCs） to differentiate into cardiomyo- cyte-like cells in vitro. The second or third generation of rBMSCs were randomly divided into the cyclic stretching stain group, the control group and the blank group. Those rBMSCs in the cyclic stretching strain group were seeded on a silicone membrane with complete medium were exposed to biaxial stretching strain of 10% of membrane at a fre- quency of 1 Hz lasting for 6 h, 12 h and 24 h. Those in the control group were seeded on silicone membrane with complete medium. Those in the blank group were seeded in the 6-wells plates with complete medium. The mRNA expression of GATA4 and myocyte-specific enhancer factor 2C （MEF-2C） were detected by the real-time fluorescent quantification PCR and the protein expression of connexin 43 （Cx43） was detected by using the Western blot meth- od. The results showed that the mRNA expression level of the GATA4 and MEF-2C, and the protein expression lev el of Cx43 were significantly higher in the cyclic stretching strain groups, compared with those in the relative control groups （P〈0. 05）. It suggests that cyclic biaxial stretching strain could play a part in the induction of rBMSCs to differentiate into cardiomyocyte-like cells in vitro, but the differentiation mechanism is still unclear.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2014年第3期596-600,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(10972149
11272225
11072163)
关键词
大鼠骨髓间充质干细胞
周期性双轴拉伸应变
心肌样细胞
rat bone marrow-derived mesenchymal stem cell
cyclic biaxial stretching strain
cardiomyocyte-like cell
